Journal article
Lipidomic scanning of self-lipids identifies headless antigens for natural killer T cells
Tan-Yun Cheng, T Praveena, Srinath Govindarajan, Catarina F Almeida, Daniel G Pellicci, Wellington C Arkins, Ildiko Van Rhijn, Koen Venken, Dirk Elewaut, Dale I Godfrey, Jamie Rossjohn, D Branch Moody
Proceedings of the National Academy of Sciences of the United States of America | National Academy of Sciences | Published : 2024
Abstract
To broadly measure the spectrum of cellular self-antigens for natural killer T cells (NKT), we developed a sensitive lipidomics system to analyze lipids trapped between CD1d and NKT T cell receptors (TCRs). We captured diverse antigen complexes formed in cells from natural endogenous lipids, with or without inducing endoplasmic reticulum (ER) stress. After separating protein complexes with no, low, or high CD1d-TCR interaction, we eluted lipids to establish the spectrum of self-lipids that facilitate this interaction. Although this unbiased approach identified fifteen molecules, they clustered into only two related groups: previously known phospholipid antigens and unexpected neutral lipid a..
View full abstractRelated Projects (5)
Grants
Awarded by DHAC | National Health and Medical Research Council (NHMRC)
Awarded by Stichting Tegen Kanker (Fondation Contre le Cancer)
Awarded by HHS | NIH (NIH)
Awarded by Research Foundation Flanders
Awarded by Australian Research Council
Awarded by CSL Limited (CSL)
Awarded by Wellcome Trust (WT)
Awarded by NIAID NIH HHS
Awarded by NIAMS NIH HHS